PrimePCR™ SYBR® Green Assay: CU915260.1, Mouse

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best efficiency; Same primer pair as used in probe assay qMmuCEP0062694

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: CU915260.1, Mouse
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: CU915260.1, Mouse
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qMmuCED0061545
Assay Design:   exonic
Chromosome Location:   X:3076946-3077077question
Amplicon Length:   102
Splice Variants Targeted:   ENSMUST00000178827 ENSMUST00000178080 ENSMUST00000105020 ENSMUST00000105019 ENSMUST00000178621 ENSMUST00000105017 ENSMUST00000179532 ENSMUST00000180197 ENSMUST00000178444 ENSMUST00000179086 ENSMUST00000178747 ENSMUST00000179991 ENSMUST00000178070 ENSMUST00000179585 ENSMUST00000179069 ENSMUST00000179538 ENSMUST00000180039 ENSMUST00000105119 ENSMUST00000177912 ENSMUST00000105117 ENSMUST00000179466 ENSMUST00000178219 ENSMUST00000180004 ENSMUST00000140300 ENSMUST00000105015 ENSMUST00000179325 ENSMUS

Gene Information

Description Not Available

Gene Symbol:   CU915260.1
Gene Name:   Not available
Aliases:   Not Available
RefSeq:   Not Available
Ensembl:   ENSMUSG00000095801
Entrez:   0
Chromosome Mapping:   Not Available

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.998800
y-intercept 36.830000
Efficiency 103

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download