PrimePCR™ SYBR® Green Assay: Lrch4, Mouse

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qMmuCEP0062639

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: Lrch4, Mouse
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: Lrch4, Mouse
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qMmuCED0061490
Assay Design:   exonic
Chromosome Location:   5:137640813-137640929question
Amplicon Length:   87
Splice Variants Targeted:   ENSMUST00000168693 ENSMUST00000163754 ENSMUST00000041609 ENSMUST00000166772 ENSMUST00000167227 ENSMUST00000095551 ENSMUST00000073094 ENSMUST00000064821 ENSMUST00000126475 ENSMUST00000106242 ENSMUST00000031734 ENSMUST00000176667 ENSMUST00000147272 ENSMUST00000095087 ENSMUST00000091146 ENSMUST00000102872 ENSMUST00000147488 ENSMUST00000020982 ENSMUST00000146894

Gene Information

Description Not Available

Gene Symbol:   Lrch4
Gene Name:   Leucine-rich repeats and calponin homology (CH) domain containing 4
Aliases:   2810008P14Rik, 2900069C24Rik, AI558103, LRN, LRRN4, SAP25, mFLJ00248
RefSeq:   NC_000071.6 NT_039315.8
Ensembl:   ENSMUSG00000093445
Entrez:   231798
Chromosome Mapping:   5 G2|5

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999100
y-intercept 35.850000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download