PrimePCR™ SYBR® Green Assay: Rab9, Mouse

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qMmuCEP0060662

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: Rab9, Mouse
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: Rab9, Mouse
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qMmuCED0052238
Assay Design:   exonic
Chromosome Location:   X:166462329-166479636question
Amplicon Length:   81
Splice Variants Targeted:   ENSMUST00000108449 ENSMUST00000063956 ENSMUST00000021888 ENSMUST00000125192 ENSMUST00000102729 ENSMUST00000175776 ENSMUST00000132165 ENSMUST00000030281 ENSMUST00000048892 ENSMUST00000102582 ENSMUST00000031583 ENSMUST00000112091 ENSMUST00000049435 ENSMUST00000108239 ENSMUST00000017694 ENSMUST00000131252 ENSMUST00000029061 ENSMUST00000085591 ENSMUST00000058033

Gene Information

Description Not Available

Gene Symbol:   Rab9
Gene Name:   RAB9, member RAS oncogene family
Aliases:   2410064E05Rik, AI195561, Rab9a, Sid6061p
RefSeq:   NC_000086.7 NT_039718.8
Ensembl:   ENSMUSG00000079316
Entrez:   56382
Chromosome Mapping:   X F5|X

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999800
y-intercept 35.910000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download