PrimePCR™ SYBR® Green Assay: Sepp1, Mouse

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage; Same primer pair as used in probe assay qMmuCEP0060264

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: Sepp1, Mouse
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: Sepp1, Mouse
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qMmuCED0051838
Assay Design:   exonic
Chromosome Location:   15:3274743-3274858question
Amplicon Length:   86
Splice Variants Targeted:   ENSMUST00000160787 ENSMUST00000159158 ENSMUST00000159216 ENSMUST00000160311 ENSMUST00000160930 ENSMUST00000082424

Gene Information

This gene encodes a selenoprotein containing multiple selenocysteine (Sec) residues encoded by the UGA codon that normally signals translation termination. The 3' UTR of selenoprotein genes have a common stem-loop structure the sec insertion sequence (SECIS) that is necessary for the recognition of UGA as a Sec codon rather than as a stop signal. This selenoprotein is a secreted protein and is unusual in that it contains 10 selenocysteine residues per polypeptide constituting most of the selenium in plasma. It has been implicated as an extracellular antioxidant and in the transport of selenium. Several transcript variants differing in the 5' UTR but encoding the same protein have been found for this gene. [provided by RefSeq Jul 2008]

Gene Symbol:   Sepp1
Gene Name:   Selenoprotein P, plasma, 1
Aliases:   AU018766, D15Ucla1, Se-P, selp
RefSeq:   NC_000081.6 NT_039617.8
Ensembl:   ENSMUSG00000064373
Entrez:   20363
Chromosome Mapping:   15 A1|15 5.9 cM

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 35.550000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download