PrimePCR™ SYBR® Green Assay: Jmjd6, Mouse

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage; Same primer pair as used in probe assay qMmuCEP0058875

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: Jmjd6, Mouse
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: Jmjd6, Mouse
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qMmuCED0050449
Assay Design:   exonic
Chromosome Location:   11:116837629-116838991question
Amplicon Length:   107
Splice Variants Targeted:   ENSMUST00000047616 ENSMUST00000124831

Gene Information

This gene encodes a nuclear protein with a JmjC domain. JmjC domain-containing proteins are predicted to function as protein hydroxylases or histone demethylases. This protein functions in differentiation of multiple tissues during development and in anti-inflammatory cytokine signaling. It was first identified as a putative phosphatidylserine receptor involved in phagocytosis of apoptotic cells; however subsequent studies have indicated that this protein does not directly function in the clearance of apoptotic cells and questioned whether it is a true phosphatidylserine receptor. [provided by RefSeq Jul 2008]

Gene Symbol:   Jmjd6
Gene Name:   Jumonji domain containing 6
Aliases:   5730436I23Rik, D11Ertd195e, PSR, PtdSerR, Ptdsr, mKIAA0585
RefSeq:   NC_000077.6 NT_096135.6
Ensembl:   ENSMUSG00000056962
Entrez:   107817
Chromosome Mapping:   11 E2|11 75.0 cM

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 35.700000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download