PrimePCR™ SYBR® Green Assay: Cyp4a31, Mouse

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage; Same primer pair as used in probe assay qMmuCEP0056554

List Price:    $146.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qMmuCED0048128
Assay Design:   exonic
Chromosome Location:   4:115570197-115570319question
Amplicon Length:   93
Splice Variants Targeted:   ENSMUST00000058785 ENSMUST00000094886 ENSMUST00000126645 ENSMUST00000030480 ENSMUST00000030486 ENSMUST00000084342

Gene Information

Description Not Available

Gene Symbol:   Cyp4a31
Gene Name:   Cytochrome P450, family 4, subfamily a, polypeptide 31
Aliases:   RP23-118K16.4
RefSeq:   NC_000070.6 NT_039264.7
Ensembl:   ENSMUSG00000028712
Entrez:   666168
UniGene:   Mm.482086
Chromosome Mapping:   4 D1|4

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 35.840000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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