PrimePCR™ SYBR® Green Assay: Aip, Mouse

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage; Same primer pair as used in probe assay qMmuCEP0055221

List Price:    $174.00
Your Price:   Log In
Quantity:  
 
Add to Custom Plate

PrimePCR™ PreAmp for SYBR® Green Assay: Aip, Mouse
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: Aip, Mouse
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qMmuCED0046795
Assay Design:   exonic
Chromosome Location:   19:4121412-4125741question
Amplicon Length:   116
Splice Variants Targeted:   ENSMUST00000114017 ENSMUST00000114015 ENSMUST00000114013 ENSMUST00000077355 ENSMUST00000114012 ENSMUST00000114018 ENSMUST00000024639 ENSMUST00000078470 ENSMUST00000110844 ENSMUST00000117831 ENSMUST00000025767 ENSMUST00000121402 ENSMUST00000173199 ENSMUST00000018087 ENSMUST00000109420 ENSMUST00000156000 ENSMUST00000093166 ENSMUST00000093165 ENSMUST00000165599 ENSMUST00000106677 ENSMUST00000049527 ENSMUST00000112121 ENSMUST00000053271 ENSMUST00000112242 ENSMUST00000164034 ENSMUST00000020101 ENSMUS

Gene Information

Description Not Available

Gene Symbol:   Aip
Gene Name:   Aryl-hydrocarbon receptor-interacting protein
Aliases:   AA408703, AW476050, Ara9, D19Bwg1412e, Fkbp16, Xap2
RefSeq:   NC_000085.6 NT_082868.6 NW_004058054.1
Ensembl:   ENSMUSG00000024847
Entrez:   11632
Chromosome Mapping:   19 A|19 0.0 cM

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 35.890000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download