PrimePCR™ SYBR® Green Assay: Tef, Mouse

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage; Same primer pair as used in probe assay qMmuCEP0055206

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: Tef, Mouse
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: Tef, Mouse
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qMmuCED0046780
Assay Design:   exonic
Chromosome Location:   15:81814956-81815072question
Amplicon Length:   87
Splice Variants Targeted:   ENSMUST00000108255 ENSMUST00000099163 ENSMUST00000177992 ENSMUST00000033050 ENSMUST00000114671 ENSMUST00000114680 ENSMUST00000114669 ENSMUST00000028076 ENSMUST00000162075 ENSMUST00000109553 ENSMUST00000168200 ENSMUST00000023024 ENSMUST00000172208 ENSMUST00000129404 ENSMUST00000105426 ENSMUST00000131321 ENSMUST00000126920 ENSMUST00000147189 ENSMUST00000105424 ENSMUST00000092420 ENSMUST00000105429 ENSMUST00000131724 ENSMUST00000152655 ENSMUST00000151116 ENSMUST00000155701 ENSMUST00000152819 ENSMUS

Gene Information

Description Not Available

Gene Symbol:   Tef
Gene Name:   Thyrotroph embryonic factor
Aliases:   2310028D20Rik
RefSeq:   NC_000081.6 NT_039621.8
Ensembl:   ENSMUSG00000022389
Entrez:   21685
Chromosome Mapping:   15 E1|15 46.7 cM

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 36.250000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download