PrimePCR™ SYBR® Green Assay: Hmg20b, Mouse

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage; Same primer pair as used in probe assay qMmuCEP0053871

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: Hmg20b, Mouse
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: Hmg20b, Mouse
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qMmuCED0045445
Assay Design:   exonic
Chromosome Location:   10:81348532-81348909question
Amplicon Length:   79
Splice Variants Targeted:   ENSMUST00000114870 ENSMUST00000025054 ENSMUST00000167489 ENSMUST00000120223 ENSMUST00000097655 ENSMUST00000035202 ENSMUST00000105323 ENSMUST00000122993 ENSMUST00000020454 ENSMUST00000105324 ENSMUST00000141171 ENSMUST00000154609 ENSMUST00000167481 ENSMUST00000136789 ENSMUST00000112819 ENSMUST00000057631 ENSMUST00000081799 ENSMUST00000159976 ENSMUST00000107618 ENSMUST00000159212 ENSMUST00000160260 ENSMUST00000111429 ENSMUST00000160908 ENSMUST00000027850 ENSMUST00000069609 ENSMUST00000176942 ENSMUS

Gene Information

Description Not Available

Gene Symbol:   Hmg20b
Gene Name:   High mobility group 20 B
Aliases:   AW610687, BRAF35, Hmgx2, Hmgxb2, Smarce1r
RefSeq:   NC_000076.6 NT_039500.8
Ensembl:   ENSMUSG00000020232
Entrez:   15353
Chromosome Mapping:   10 C1|10 43.0 cM

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.998500
y-intercept 34.790000
Efficiency 103

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download