PrimePCR™ SYBR® Green Assay: Obox5, Mouse

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qMmuCEP0043568

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: Obox5, Mouse
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: Obox5, Mouse
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qMmuCED0041352
Assay Design:   exonic
Chromosome Location:   7:15757952-15758077question
Amplicon Length:   96
Splice Variants Targeted:   ENSMUST00000069740 ENSMUST00000172478 ENSMUST00000036575 ENSMUST00000174076 ENSMUST00000174305 ENSMUST00000172881 ENSMUST00000067288 ENSMUST00000172463 ENSMUST00000173443 ENSMUST00000173245 ENSMUST00000181001 ENSMUST00000174443 ENSMUST00000173912 ENSMUST00000173395 ENSMUST00000174151 ENSMUST00000174842 ENSMUST00000086122 ENSMUST00000095217 ENSMUST00000173455 ENSMUST00000098802 ENSMUST00000173053

Gene Information

Description Not Available

Gene Symbol:   Obox5
Gene Name:   Oocyte specific homeobox 5
Aliases:   C87544
RefSeq:   NC_000073.6 NT_187034.1
Ensembl:   ENSMUSG00000074366
Entrez:   252829
Chromosome Mapping:   7 A2|7

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999900
y-intercept 39.330000
Efficiency 88

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download