PrimePCR™ SYBR® Green Assay: Sft2d1, Mouse

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qMmuCEP0043541

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: Sft2d1, Mouse
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: Sft2d1, Mouse
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qMmuCED0041325
Assay Design:   exonic
Chromosome Location:   17:8311188-8311282question
Amplicon Length:   65
Splice Variants Targeted:   ENSMUST00000065112 ENSMUST00000110081 ENSMUST00000110078 ENSMUST00000093823 ENSMUST00000108492 ENSMUST00000105902 ENSMUST00000105900 ENSMUST00000097929 ENSMUST00000027521 ENSMUST00000074945 ENSMUST00000105369 ENSMUST00000170219 ENSMUST00000169546 ENSMUST00000109736 ENSMUST00000065049 ENSMUST00000128972 ENSMUST00000147812 ENSMUST00000109738 ENSMUST00000063289 ENSMUST00000021339 ENSMUST00000002850 ENSMUST00000102801 ENSMUST00000057207 ENSMUST00000163524 ENSMUST00000154553 ENSMUST00000093169 ENSMUS

Gene Information

Description Not Available

Gene Symbol:   Sft2d1
Gene Name:   SFT2 domain containing 1
Aliases:   5630401J11Rik, AA409698
RefSeq:   NC_000083.6 NT_039638.8
Ensembl:   ENSMUSG00000073468
Entrez:   106489
Chromosome Mapping:   17 A1|17

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 42.320000
Efficiency 80

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download