PrimePCR™ SYBR® Green Assay: Speer4c, Mouse

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qMmuCEP0043534

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: Speer4c, Mouse
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: Speer4c, Mouse
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qMmuCED0041318
Assay Design:   exonic
Chromosome Location:   5:15709744-15709840question
Amplicon Length:   67
Splice Variants Targeted:   ENSMUST00000168875 ENSMUST00000079447 ENSMUST00000094946 ENSMUST00000088236 ENSMUST00000074148 ENSMUST00000053257 ENSMUST00000166086 ENSMUST00000076099 ENSMUST00000095015 ENSMUST00000168057 ENSMUST00000170064 ENSMUST00000163544 ENSMUST00000170260 ENSMUST00000171093 ENSMUST00000168407 ENSMUST00000164262 ENSMUST00000172106 ENSMUST00000167041 ENSMUST00000164651 ENSMUST00000088616 ENSMUST00000170057 ENSMUST00000167566 ENSMUST00000168154 ENSMUST00000168329 ENSMUST00000170301 ENSMUST00000124677 ENSMUS

Gene Information

Description Not Available

Gene Symbol:   Speer4c
Gene Name:   Spermatogenesis associated glutamate (E)-rich protein 4c
Aliases:   4933431D05Rik, SPEER-4C
RefSeq:   NC_000071.6 NT_165760.3
Ensembl:   ENSMUSG00000073208
Entrez:   100502967
Chromosome Mapping:   5 A2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.997400
y-intercept 37.300000
Efficiency 95

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download