PrimePCR™ SYBR® Green Assay: Rpl9, Mouse

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qMmuCEP0043175

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: Rpl9, Mouse
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: Rpl9, Mouse
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qMmuCED0040958
Assay Design:   exonic
Chromosome Location:   5:65390415-65390763question
Amplicon Length:   63
Splice Variants Targeted:   ENSMUST00000035148 ENSMUST00000002176 ENSMUST00000114934 ENSMUST00000114927 ENSMUST00000142941 ENSMUST00000114924 ENSMUST00000100429 ENSMUST00000068767 ENSMUST00000119386 ENSMUST00000031090 ENSMUST00000060989 ENSMUST00000057885 ENSMUST00000120094 ENSMUST00000118543 ENSMUST00000127874 ENSMUST00000045521 ENSMUST00000137768 ENSMUST00000081162 ENSMUST00000142283 ENSMUST00000047923 ENSMUST00000027690 ENSMUST00000025831 ENSMUST00000070203 ENSMUST00000127825 ENSMUST00000041920 ENSMUST00000052224 ENSMUS

Gene Information

Description Not Available

Gene Symbol:   Rpl9
Gene Name:   Ribosomal protein L9
Aliases:   Not Available
RefSeq:   NC_000071.6 NT_039305.8
Ensembl:   ENSMUSG00000047215
Entrez:   20005
Chromosome Mapping:   5 C3.1|5

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 36.540000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download