PrimePCR™ SYBR® Green Assay: Rell2, Mouse

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage; Same primer pair as used in probe assay qMmuCEP0043156

List Price:    $174.00
Your Price:   Log In
Quantity:  
 
Add to Custom Plate

PrimePCR™ PreAmp for SYBR® Green Assay: Rell2, Mouse
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: Rell2, Mouse
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qMmuCED0040939
Assay Design:   exonic
Chromosome Location:   18:37957589-37957678question
Amplicon Length:   60
Splice Variants Targeted:   ENSMUST00000136755 ENSMUST00000078163 ENSMUST00000179893 ENSMUST00000032813 ENSMUST00000176902 ENSMUST00000176104 ENSMUST00000070709 ENSMUST00000177058 ENSMUST00000169360 ENSMUST00000163591 ENSMUST00000091932 ENSMUST00000169498 ENSMUST00000043437 ENSMUST00000095446 ENSMUST00000178075 ENSMUST00000085044 ENSMUST00000166103 ENSMUST00000006854 ENSMUST00000057846 ENSMUST00000126927 ENSMUST00000112370 ENSMUST00000144548 ENSMUST00000159038 ENSMUST00000112365 ENSMUST00000034457 ENSMUST00000113504 ENSMUS

Gene Information

Description Not Available

Gene Symbol:   Rell2
Gene Name:   RELT-like 2
Aliases:   4631403P03Rik, ependolin
RefSeq:   NC_000084.6 NT_039674.8
Ensembl:   ENSMUSG00000044024
Entrez:   225392
Chromosome Mapping:   18 B3|18

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999300
y-intercept 36.900000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download