PrimePCR™ SYBR® Green Assay: Ost4, Mouse

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qMmuCEP0043132

List Price:    $146.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qMmuCED0040915
Assay Design:   exonic
Chromosome Location:   5:30906665-30906757question
Amplicon Length:   63
Splice Variants Targeted:   ENSMUST00000031057 ENSMUST00000132253 ENSMUST00000132034

Gene Information

Description Not Available

Gene Symbol:   Ost4
Gene Name:   Oligosaccharyltransferase 4 homolog (S. cerevisiae)
Aliases:   2310016E02Rik
RefSeq:   NC_000071.6 NT_039305.8
Ensembl:   ENSMUSG00000038803
Entrez:   67695
UniGene:   Mm.21450
Chromosome Mapping:   5 B1|5

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999300
y-intercept 37.100000
Efficiency 92

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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