PrimePCR™ SYBR® Green Assay: Psmd11, Mouse

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qMmuCEP0043019

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: Psmd11, Mouse
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: Psmd11, Mouse
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qMmuCED0040802
Assay Design:   exonic
Chromosome Location:   11:80462611-80465043question
Amplicon Length:   60
Splice Variants Targeted:   ENSMUST00000140364 ENSMUST00000174552 ENSMUST00000169960 ENSMUST00000166299 ENSMUST00000034308 ENSMUST00000176860 ENSMUST00000114354 ENSMUST00000114352 ENSMUST00000068286 ENSMUST00000114360 ENSMUST00000033769 ENSMUST00000148317 ENSMUST00000114353 ENSMUST00000101458 ENSMUST00000138397 ENSMUST00000126583 ENSMUST00000112292 ENSMUST00000086599 ENSMUST00000161610 ENSMUST00000108775 ENSMUST00000163193 ENSMUST00000047697 ENSMUST00000020161 ENSMUST00000173938 ENSMUST00000017572 ENSMUST00000148895 ENSMUS

Gene Information

Description Not Available

Gene Symbol:   Psmd11
Gene Name:   Proteasome (prosome, macropain) 26S subunit, non-ATPase, 11
Aliases:   1700089D09Rik, 1810019E17Rik, 2610024G20Rik, 2810055C24Rik, C78232, P44.5, S9
RefSeq:   NC_000077.6 NT_096135.6
Ensembl:   ENSMUSG00000017428
Entrez:   69077
Chromosome Mapping:   11 B5|11

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 38.460000
Efficiency 90

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download