PrimePCR™ SYBR® Green Assay: U2af1l4, Mouse

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qMmuCEP0042854

List Price:    $174.00
Your Price:   Log In
Quantity:  
 
Add to Custom Plate

PrimePCR™ PreAmp for SYBR® Green Assay: U2af1l4, Mouse
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: U2af1l4, Mouse
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qMmuCED0040637
Assay Design:   exonic
Chromosome Location:   7:30564204-30564487question
Amplicon Length:   81
Splice Variants Targeted:   ENSMUST00000043273 ENSMUST00000163654 ENSMUST00000166510 ENSMUST00000163482 ENSMUST00000166257 ENSMUST00000163464 ENSMUST00000168931 ENSMUST00000163330

Gene Information

Description Not Available

Gene Symbol:   U2af1l4
Gene Name:   U2 small nuclear RNA auxiliary factor 1-like 4
Aliases:   AA407033, AF419339, AI451269, AW553050, U2af26
RefSeq:   NC_000073.6 NT_187034.1
Ensembl:   ENSMUSG00000078765
Entrez:   233073
Chromosome Mapping:   7 B1|7

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999400
y-intercept 39.040000
Efficiency 88

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download