PrimePCR™ SYBR® Green Assay: 1700029G01Rik, Mouse

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qMmuCEP0042226

List Price:    $142.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qMmuCED0040007
Assay Design:   exonic
Chromosome Location:   4:126151625-126151734question
Amplicon Length:   80
Splice Variants Targeted:   ENSMUST00000094760 ENSMUST00000097891

Gene Information

Description Not Available

Gene Symbol:   1700029G01Rik
Gene Name:   RIKEN cDNA 1700029G01 gene
Aliases:   1700029G01Rik, AU019788
RefSeq:   NC_000070.6 NT_039264.7
Ensembl:   ENSMUSG00000073758
Entrez:   66938
UniGene:   Mm.29813
Chromosome Mapping:   4 D2.2|4

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.996800
y-intercept 36.080000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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