PrimePCR™ SYBR® Green Assay: Crem, Mouse

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qMmuCEP0042209

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: Crem, Mouse
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: Crem, Mouse
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qMmuCED0039990
Assay Design:   exonic
Chromosome Location:   18:3273442-3273571question
Amplicon Length:   100
Splice Variants Targeted:   ENSMUST00000151311 ENSMUST00000150235 ENSMUST00000122958 ENSMUST00000154135 ENSMUST00000140332 ENSMUST00000154470 ENSMUST00000129435 ENSMUST00000151084 ENSMUST00000131899 ENSMUST00000130599 ENSMUST00000124747 ENSMUST00000049942 ENSMUST00000127601 ENSMUST00000147138 ENSMUST00000156234 ENSMUST00000152900 ENSMUST00000130455 ENSMUST00000137568 ENSMUST00000146265 ENSMUST00000152108 ENSMUST00000136961 ENSMUST00000144496 ENSMUST00000123672 ENSMUST00000139537 ENSMUST00000154705 ENSMUST00000126578 ENSMUS

Gene Information

This gene encodes a basic-leucine zipper domain-containing protein that localizes to gene promoters where it binds to the cyclic AMP response element (CRE). Different protein isoforms encoded by this gene may function as either activators or repressors of transcription. Activity of this gene is important in multiple developmental processes including spermatogenesis. Mutation of this gene causes male infertility. Alternative splicing and promoter usage result in multiple transcript variants for this gene. [provided by RefSeq Oct 2012]

Gene Symbol:   Crem
Gene Name:   CAMP responsive element modulator
Aliases:   ICER, ICERI
RefSeq:   NC_000084.6 NT_039674.8
Ensembl:   ENSMUSG00000063889
Entrez:   12916
Chromosome Mapping:   18|18 A

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 36.780000
Efficiency 94

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download