PrimePCR™ SYBR® Green Assay: A630033H20Rik, Mouse

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qMmuCEP0039423

List Price:    $142.00
Your Price:   Log In
 
Add to Custom Plate

Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qMmuCED0038378
Assay Design:   Exonic
Chromosome Location:   X:107170922-107171056question
Amplicon Length:   105
Splice Variants Targeted:   ENSMUST00000147521 ENSMUST00000125676 ENSMUST00000137107 ENSMUST00000067249 ENSMUST00000167673 ENSMUST00000180182

Gene Information

Description Not Available

Gene Symbol:   A630033H20Rik
Gene Name:   RIKEN cDNA A630033H20 gene
Aliases:   AI662791, P2ry10
RefSeq:   NC_000086.7 NT_039706.8
Ensembl:   ENSMUSG00000054293
Entrez:   213438
UniGene:   Mm.265244
Chromosome Mapping:   X D|X

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.998100
y-intercept 36.320000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download