PrimePCR™ SYBR® Green Assay: F12, Mouse

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qMmuCEP0039264

List Price:    $142.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qMmuCED0038219
Assay Design:   Exonic
Chromosome Location:   13:55418093-55418238question
Amplicon Length:   116
Splice Variants Targeted:   ENSMUST00000021948 ENSMUST00000170921

Gene Information

Description Not Available

Gene Symbol:   F12
Gene Name:   Coagulation factor XII (Hageman factor)
Aliases:   FXII, HAF
RefSeq:   NC_000079.6 NT_039589.8
Ensembl:   ENSMUSG00000021492
Entrez:   58992
UniGene:   Mm.42224
Chromosome Mapping:   13|13 B2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 36.690000
Efficiency 94

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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