PrimePCR™ SYBR® Green Assay: Mcfd2, Mouse

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qMmuCED0026826
Assay Design:   Exonic
Chromosome Location:   17:87255804-87255906question
Amplicon Length:   73
Splice Variants Targeted:   ENSMUST00000024963 ENSMUST00000144236 ENSMUST00000129616

Gene Information

Description Not Available

Gene Symbol:   Mcfd2
Gene Name:   Multiple coagulation factor deficiency 2
Aliases:   1810021C21Rik, F5f8d, Lman1ip, Sdnsf
RefSeq:   NC_000083.6 NT_039649.8
Ensembl:   ENSMUSG00000024150
Entrez:   193813
UniGene:   Mm.30251
Chromosome Mapping:   17 E4|17

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.998800
y-intercept 35.300000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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