PrimePCR™ SYBR® Green Assay: Romo1, Mouse

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qMmuCED0025135
Assay Design:   Exonic
Chromosome Location:   2:156145602-156145776question
Amplicon Length:   145
Splice Variants Targeted:   ENSMUST00000119950 ENSMUST00000088610 ENSMUST00000109598 ENSMUST00000109597

Gene Information

Description Not Available

Gene Symbol:   Romo1
Gene Name:   Reactive oxygen species modulator 1
Aliases:   2010100O12Rik, AI853864
RefSeq:   NC_000068.7 NT_039207.8
Ensembl:   ENSMUSG00000067847
Entrez:   67067
UniGene:   Mm.371623
Chromosome Mapping:   2 H1|2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 35.530000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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