PrimePCR™ SYBR® Green Assay: A130010J15Rik, Mouse

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qMmuCED0025074
Assay Design:   Exonic
Chromosome Location:   1:193174879-193174997question
Amplicon Length:   89
Splice Variants Targeted:   ENSMUST00000160822 ENSMUST00000161235 ENSMUST00000110831 ENSMUST00000178744

Gene Information

Description Not Available

Gene Symbol:   A130010J15Rik
Gene Name:   RIKEN cDNA A130010J15 gene
Aliases:   Not Available
RefSeq:   NC_000067.6 NT_039190.8
Ensembl:   ENSMUSG00000079144
Entrez:   319266
UniGene:   Mm.482460
Chromosome Mapping:   1 H6|1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.996900
y-intercept 34.510000
Efficiency 106

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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