PrimePCR™ SYBR® Green Assay: Mapk3, Mouse

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qMmuCED0025043
Assay Design:   Exonic
Chromosome Location:   7:126764735-126764831question
Amplicon Length:   67
Splice Variants Targeted:   ENSMUST00000091328 ENSMUST00000050201 ENSMUST00000057669

Gene Information

Description Not Available

Gene Symbol:   Mapk3
Gene Name:   Mitogen-activated protein kinase 3
Aliases:   Erk-1, Erk1, Ert2, Esrk1, Mnk1, Mtap2k, Prkm3, p44, p44erk1, p44mapk
RefSeq:   NC_000073.6 NT_039433.8
Ensembl:   ENSMUSG00000063065
Entrez:   26417
UniGene:   Mm.8385
Chromosome Mapping:   7 F3|7 61.0 cM

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999900
y-intercept 35.300000
Efficiency 101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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