PrimePCR™ SYBR® Green Assay: 2010107G23Rik, Mouse

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qMmuCED0024617
Assay Design:   Exonic
Chromosome Location:   10:62108353-62108460question
Amplicon Length:   78
Splice Variants Targeted:   ENSMUST00000125704 ENSMUST00000142796 ENSMUST00000124615 ENSMUST00000142821 ENSMUST00000064050

Gene Information

Description Not Available

Gene Symbol:   2010107G23Rik
Gene Name:   RIKEN cDNA 2010107G23 gene
Aliases:   AA545165
RefSeq:   NC_000076.6 NT_039500.8
Ensembl:   ENSMUSG00000020083
Entrez:   69894
UniGene:   Mm.294126
Chromosome Mapping:   10 B4|10

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 36.310000
Efficiency 93

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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