PrimePCR™ SYBR® Green Assay: Olfr869, Mouse

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qMmuCEP0034944

List Price:    $142.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qMmuCED0004860
Assay Design:   Exonic
Chromosome Location:   9:20137177-20137308question
Amplicon Length:   102
Splice Variants Targeted:   ENSMUST00000088687 ENSMUST00000098140 ENSMUST00000072368 ENSMUST00000092849 ENSMUST00000080461 ENSMUST00000173347 ENSMUST00000173727 ENSMUST00000173009 ENSMUST00000131537 ENSMUST00000173722 ENSMUST00000021039 ENSMUST00000058296 ENSMUST00000075717

Gene Information

Olfactory receptors interact with odorant molecules in the nose to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. [provided by RefSeq Jul 2008]

Gene Symbol:   Olfr869
Gene Name:   Olfactory receptor 869
Aliases:   MOR145-6
RefSeq:   NC_000075.6 NT_039472.8
Ensembl:   ENSMUSG00000058491
Entrez:   258550
UniGene:   Mm.334988
Chromosome Mapping:   9 A3|9

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999300
y-intercept 35.400000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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