PrimePCR™ SYBR® Green Assay: Gzmg, Mouse

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: Gzmg, Mouse
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: Gzmg, Mouse
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qMmuCED0004855
Assay Design:   Exonic
Chromosome Location:   14:56157227-56157316question
Amplicon Length:   60
Splice Variants Targeted:   ENSMUST00000084820 ENSMUST00000021078 ENSMUST00000048988 ENSMUST00000155281 ENSMUST00000128676 ENSMUST00000113974 ENSMUST00000113972 ENSMUST00000113973 ENSMUST00000176262 ENSMUST00000113977 ENSMUST00000156933 ENSMUST00000123884 ENSMUST00000113034 ENSMUST00000113033 ENSMUST00000028237 ENSMUST00000113037 ENSMUST00000077855 ENSMUST00000087050 ENSMUST00000139806 ENSMUST00000024840 ENSMUST00000164647 ENSMUST00000017900 ENSMUST00000063140 ENSMUST00000048150 ENSMUST00000125866 ENSMUST00000117282 ENSMUS

Gene Information

Description Not Available

Gene Symbol:   Gzmg
Gene Name:   Granzyme G
Aliases:   Ctla-7, Ctla7
RefSeq:   NC_000080.6 NT_039606.8
Ensembl:   ENSMUSG00000040284
Entrez:   14944
Chromosome Mapping:   14 C3|14 20.5 cM

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999400
y-intercept 34.840000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download