PrimePCR™ SYBR® Green Assay: I830012O16Rik, Mouse

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qMmuCED0004849
Assay Design:   Exonic
Chromosome Location:   19:34613246-34613384question
Amplicon Length:   109
Splice Variants Targeted:   ENSMUST00000103085 ENSMUST00000073062 ENSMUST00000043896 ENSMUST00000106267 ENSMUST00000102752 ENSMUST00000039484 ENSMUST00000120734 ENSMUST00000055931 ENSMUST00000046383 ENSMUST00000128302 ENSMUST00000076249

Gene Information

Description Not Available

Gene Symbol:   I830012O16Rik
Gene Name:   RIKEN cDNA I830012O16 gene
Aliases:   Not Available
RefSeq:   NC_000085.6 NT_039687.8
Ensembl:   ENSMUSG00000062488
Entrez:   667370
UniGene:   Mm.271850
Chromosome Mapping:   19 C1|19

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 34.810000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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