PrimePCR™ SYBR® Green Assay: 5730403B10Rik, Mouse

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qMmuCED0004708
Assay Design:   Exonic
Chromosome Location:   16:4766962-4767051question
Amplicon Length:   60
Splice Variants Targeted:   ENSMUST00000004173 ENSMUST00000118703

Gene Information

Description Not Available

Gene Symbol:   5730403B10Rik
Gene Name:   RIKEN cDNA 5730403B10 gene
Aliases:   2700048O17Rik, AU018521, AW742692, CDIP
RefSeq:   NC_000082.6 NT_039624.8
Ensembl:   ENSMUSG00000004071
Entrez:   66626
UniGene:   Mm.157648
Chromosome Mapping:   16 A1|16 2.7 cM

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999400
y-intercept 34.600000
Efficiency 103

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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