PrimePCR™ SYBR® Green Assay: Relt, Mouse

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qMmuCEP0034918

List Price:    $142.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qMmuCED0004537
Assay Design:   Exonic
Chromosome Location:   7:100851361-100851490question
Amplicon Length:   100
Splice Variants Targeted:   ENSMUST00000008462 ENSMUST00000156855 ENSMUST00000139604 ENSMUST00000136231

Gene Information

Description Not Available

Gene Symbol:   Relt
Gene Name:   RELT tumor necrosis factor receptor
Aliases:   E430021K24Rik, Tnfrsf19l
RefSeq:   NC_000073.6 NT_039433.8
Ensembl:   ENSMUSG00000008318
Entrez:   320100
UniGene:   Mm.40336
Chromosome Mapping:   7 E3|7

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999900
y-intercept 35.460000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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