PrimePCR™ SYBR® Green Assay: B020031M17Rik, Mouse

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qMmuCEP0034757

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: B020031M17Rik, Mouse
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: B020031M17Rik, Mouse
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qMmuCED0004467
Assay Design:   Exonic
Chromosome Location:   13:119949628-119949815question
Amplicon Length:   158
Splice Variants Targeted:   ENSMUST00000100069 ENSMUST00000066432 ENSMUST00000170872 ENSMUST00000108828 ENSMUST00000169033 ENSMUST00000086521 ENSMUST00000179131 ENSMUST00000178510 ENSMUST00000179502 ENSMUST00000080505 ENSMUST00000179071 ENSMUST00000178828 ENSMUST00000177561 ENSMUST00000179905 ENSMUST00000081558 ENSMUST00000178349 ENSMUST00000030187 ENSMUST00000087541 ENSMUST00000113252 ENSMUST00000044507 ENSMUST00000110018 ENSMUST00000105650 ENSMUST00000055226 ENSMUST00000176652 ENSMUST00000021056 ENSMUST00000159861 ENSMUS

Gene Information

Description Not Available

Gene Symbol:   B020031M17Rik
Gene Name:   RIKEN cDNA B020031M17 gene
Aliases:   Not Available
RefSeq:   Not Available
Ensembl:   ENSMUSG00000078537
Chromosome Mapping:   Not Available

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 35.650000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download