PrimePCR™ SYBR® Green Assay: Ccdc85b, Mouse

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qMmuCEP0034750

List Price:    $142.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qMmuCED0004460
Assay Design:   Exonic
Chromosome Location:   19:5456941-5457166question
Amplicon Length:   196
Splice Variants Targeted:   ENSMUST00000064571 ENSMUST00000113294 ENSMUST00000179549 ENSMUST00000100036 ENSMUST00000041124 ENSMUST00000125366 ENSMUST00000117721 ENSMUST00000055369 ENSMUST00000180062 ENSMUST00000177608 ENSMUST00000088585 ENSMUST00000053426 ENSMUST00000119015

Gene Information

Description Not Available

Gene Symbol:   Ccdc85b
Gene Name:   Coiled-coil domain containing 85B
Aliases:   AI842788, BB115872
RefSeq:   NC_000085.6 NT_082868.6
Ensembl:   ENSMUSG00000095098
Entrez:   240514
UniGene:   Mm.329657
Chromosome Mapping:   19 A|19

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 35.180000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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