PrimePCR™ SYBR® Green Assay: Gm10033, Mouse

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qMmuCED0004414
Assay Design:   Exonic
Chromosome Location:   8:69373252-69373355question
Amplicon Length:   74
Splice Variants Targeted:   ENSMUST00000109051 ENSMUST00000109020 ENSMUST00000108959 ENSMUST00000128657 ENSMUST00000119838 ENSMUST00000108942 ENSMUST00000099418 ENSMUST00000073410 ENSMUST00000110214 ENSMUST00000107992 ENSMUST00000174558 ENSMUST00000163106 ENSMUST00000072427

Gene Information

Description Not Available

Gene Symbol:   Gm10033
Gene Name:   Predicted gene 10033
Aliases:   Not Available
RefSeq:   Not Available
Ensembl:   ENSMUSG00000057924
UniGene:   Mm.37846
Chromosome Mapping:   Not Available

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999800
y-intercept 35.840000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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