PrimePCR™ SYBR® Green Assay: Gjd3, Mouse

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qMmuCED0004395
Assay Design:   Exonic
Chromosome Location:   11:98982704-98982801question
Amplicon Length:   68
Splice Variants Targeted:   ENSMUST00000062931 ENSMUST00000028170 ENSMUST00000113893 ENSMUST00000100241 ENSMUST00000140704 ENSMUST00000148287 ENSMUST00000075540

Gene Information

Description Not Available

Gene Symbol:   Gjd3
Gene Name:   Gap junction protein, delta 3
Aliases:   Gja11, Gjc1, cx30.2
RefSeq:   NC_000077.6 NT_096135.6
Ensembl:   ENSMUSG00000047197
Entrez:   353155
UniGene:   Mm.453253
Chromosome Mapping:   11 D|11

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.998800
y-intercept 36.050000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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