PrimePCR™ SYBR® Green Assay: Olfr179, Mouse

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qMmuCEP0034565

List Price:    $142.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qMmuCED0004370
Assay Design:   Exonic
Chromosome Location:   11:58666067-58666171question
Amplicon Length:   75
Splice Variants Targeted:   ENSMUST00000162505 ENSMUST00000037912 ENSMUST00000072030 ENSMUST00000078217 ENSMUST00000057453 ENSMUST00000076623 ENSMUST00000160262 ENSMUST00000095729 ENSMUST00000100530 ENSMUST00000161279

Gene Information

Olfactory receptors interact with odorant molecules in the nose to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. [provided by RefSeq Jul 2008]

Gene Symbol:   Olfr179
Gene Name:   Olfactory receptor 179
Aliases:   GA_x5J8B7U1E65-1-838, MOR256-64, OTTMUSG00000005755, Olfr179-ps1, Olfr216, Olfr216-ps1
RefSeq:   NC_000077.6 NT_078683.1 NT_096135.6
Ensembl:   ENSMUSG00000063549
Entrez:   404346
UniGene:   Mm.439635
Chromosome Mapping:   11 B1.3|11

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 35.700000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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