PrimePCR™ SYBR® Green Assay: Rps13, Mouse

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qMmuCED0004332
Assay Design:   Exonic
Chromosome Location:   7:116334085-116334190question
Amplicon Length:   76
Splice Variants Targeted:   ENSMUST00000094227 ENSMUST00000030305 ENSMUST00000037696 ENSMUST00000110837 ENSMUST00000099546 ENSMUST00000170953 ENSMUST00000134935 ENSMUST00000043108 ENSMUST00000040523 ENSMUST00000068240 ENSMUST00000068259 ENSMUST00000031798 ENSMUST00000036951 ENSMUST00000044503 ENSMUST00000113195 ENSMUST00000159109 ENSMUST00000023509 ENSMUST00000050003 ENSMUST00000133191 ENSMUST00000167320 ENSMUST00000178486 ENSMUST00000023741 ENSMUST00000072607

Gene Information

Description Not Available

Gene Symbol:   Rps13
Gene Name:   Ribosomal protein S13
Aliases:   2700063M04Rik
RefSeq:   NC_000073.6 NT_039433.8
Ensembl:   ENSMUSG00000090862
Entrez:   68052
UniGene:   Mm.14798
Chromosome Mapping:   7|7 E1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 34.620000
Efficiency 104

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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