PrimePCR™ SYBR® Green Assay: Cox8c, Mouse

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qMmuCEP0028046

List Price:    $142.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qMmuCED0004126
Assay Design:   Exonic
Chromosome Location:   12:102900391-102900508question
Amplicon Length:   88
Splice Variants Targeted:   ENSMUST00000053611

Gene Information

Description Not Available

Gene Symbol:   Cox8c
Gene Name:   Cytochrome c oxidase, subunit VIIIc
Aliases:   1700007F21Rik, COX8-3, COXVIII-3
RefSeq:   NC_000078.6 NT_039551.8
Ensembl:   ENSMUSG00000043319
Entrez:   75483
UniGene:   Mm.660
Chromosome Mapping:   12 E|12

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 35.550000
Efficiency 95

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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