PrimePCR™ SYBR® Green Assay: Rpl22l1, Mouse

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qMmuCEP0028008

List Price:    $142.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qMmuCED0003961
Assay Design:   Exonic
Chromosome Location:   3:28807248-69898864question
Amplicon Length:   92
Splice Variants Targeted:   ENSMUST00000043867

Gene Information

Description Not Available

Gene Symbol:   Rpl22l1
Gene Name:   Ribosomal protein L22 like 1
Aliases:   3110001N18Rik, AU041196
RefSeq:   NC_000069.6 NT_162143.4
Ensembl:   ENSMUSG00000039221
Entrez:   68028
UniGene:   Mm.371630
Chromosome Mapping:   3 A3|3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.997500
y-intercept 34.820000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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