PrimePCR™ SYBR® Green Assay: Prdx6b, Mouse

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

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PrimePCR™ PreAmp for SYBR® Green Assay: Prdx6b, Mouse
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: Prdx6b, Mouse
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qMmuCED0002120
Assay Design:   Exonic
Chromosome Location:   2:80292489-80292599question
Amplicon Length:   81
Splice Variants Targeted:   ENSMUST00000057072

Gene Information

This intronless gene is similar to the multi-exon peroxiredoxin 6 gene located on chromosome 1. It is transcribed and the ORF is intact compared to that of the peroxiredoxin 6 gene. This gene could be considered a transcribed pseudogene based on a failure to detect the protein in vivo in PMID:14644414. However NCBI is representing the protein due to mass spectrometry data in PMID:18614015 which detected at least one peptide that is specific for this protein. [provided by RefSeq Mar 2011]

Gene Symbol:   Prdx6b
Gene Name:   Peroxiredoxin 6B
Aliases:   1-cysPrx-P1, 4930414C22Rik, Aop2-rs1, Prdx5-rs1, Prdx6-ps1, Prdx6-rs1, Prdx6-rs1-ps
RefSeq:   NC_000068.7 NT_039207.8
Ensembl:   ENSMUSG00000050114
Entrez:   320769
Chromosome Mapping:   2 C3|2 47.5 cM

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.998700
y-intercept 35.960000
Efficiency 92

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download