PrimePCR™ lncRNA SYBR® Green Assay:NEK3, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression.

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qhsaLIP0210804

List Price:    $169.00
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PrimePCR™ IncRNA PreAmp for SYBR® Green Assay: NEK3, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ IncRNA Template for SYBR® Green Assay: NEK3, Human
Reaction:     4 billion copies/200 µl desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   lncRNA Expression
Unique Assay ID:   qhsaLID0133564
Assay Design:   intron_spanning
Chromosome Location:   chr13:52151185-52152644question
Amplicon Length:   120
Refseq Transcripts Amplified:   NM_002498,NM_152720
Splice Variants Targeted:   ENST00000617054 ENST00000552973 ENST00000611833 ENST00000610828 ENST00000620675 ENST00000551355 ENST00000618534
LNCipedia Splice Variants Targeted:   lnc-NEK5-3:1

Gene Information

This gene encodes a member of the NimA (never in mitosis A) family of serine/threonine protein kinases. The encoded protein differs from other NimA family members in that it is not cell cycle regulated and is found primarily in the cytoplasm. The kinase is activated by prolactin stimulation leading to phosphorylation of VAV2 guanine nucleotide exchange factor paxillin and activation of the RAC1 GTPase. Multiple transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq Mar 2009]

Gene Symbol:   NEK3
Gene Name:   NIMA (never in mitosis gene a)-related kinase 3
Aliases:   HSPK36, MGC29949
RefSeq:   NC_000013.10 NT_024524.14
Ensembl:   ENSG00000136098
LNCipedia:   lnc-NEK5-3
Entrez:   4752
Chromosome Mapping:   13q14.13
Gene Strand:   Not Available

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10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download