PrimePCR™ lncRNA SYBR® Green Assay:CETN3, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression.

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qhsaLIP0210665

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PrimePCR™ IncRNA PreAmp for SYBR® Green Assay: CETN3, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ IncRNA Template for SYBR® Green Assay: CETN3, Human
Reaction:     4 billion copies/200 µl desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   lncRNA Expression
Unique Assay ID:   qhsaLID0133425
Assay Design:   intron_spanning
Chromosome Location:   chr5:90405710-90407759question
Amplicon Length:   120
Refseq Transcripts Amplified:   NM_001297768,NM_004365,NM_001297765
Splice Variants Targeted:   ENST00000283122 ENST00000522083 ENST00000522565 ENST00000518458 ENST00000522864 ENST00000522842
LNCipedia Splice Variants Targeted:   lnc-MBLAC2-1:1

Gene Information

The protein encoded by this gene contains four EF-hand calcium binding domains and is a member of the centrin protein family. Centrins are evolutionarily conserved proteins similar to the CDC31 protein of S. cerevisiae. Yeast CDC31 is located at the centrosome of interphase and mitotic cells where it plays a fundamental role in centrosome duplication and separation. Multiple forms of the proteins similar to the yeast centrin have been identified in human and other mammalian cells some of which have been shown to be associated with centrosome fractions. This protein appears to be one of the most abundant centrins associated with centrosome which suggests a similar function to its yeast counterpart. [provided by RefSeq Jul 2008]

Gene Symbol:   CETN3
Gene Name:   centrin, EF-hand protein, 3
Aliases:   CEN3, MGC12502, MGC138245
RefSeq:   NC_000005.9 NT_006713.15
Ensembl:   ENSG00000153140
LNCipedia:   lnc-MBLAC2-1
Entrez:   1070
Chromosome Mapping:   5q14.3
Gene Strand:   Not Available

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10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download