This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
Info: Same primer pair as used in probe assay qhsaLEP0238970. May amplify unspliced transcripts of an antisense gene.
PrimePCR™ IncRNA PreAmp for SYBR® Green Assay: RFX2, Human
PrimePCR™ IncRNA Template for SYBR® Green Assay: RFX2, Human
This gene is a member of the regulatory factor X gene family which encodes transcription factors that contain a highly-conserved winged helix DNA binding domain. The protein encoded by this gene is structurally related to regulatory factors X1 X3 X4 and X5. It is a transcriptional activator that can bind DNA as a monomer or as a heterodimer with other RFX family members. This protein can bind to cis elements in the promoter of the IL-5 receptor alpha gene. Two transcript variants encoding different isoforms have been described for this gene and both variants utilize alternative polyadenylation sites. [provided by RefSeq Jul 2008]