PrimePCR™ lncRNA SYBR® Green Assay:FADS1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression.

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qhsaLEP0234752. May amplify unspliced transcripts of an antisense gene.

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PrimePCR™ IncRNA PreAmp for SYBR® Green Assay: FADS1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ IncRNA Template for SYBR® Green Assay: FADS1, Human
Reaction:     4 billion copies/200 µl desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   lncRNA Expression
Unique Assay ID:   qhsaLED0221494
Assay Design:   exonic
Chromosome Location:   chr11:61814403-61814573question
Amplicon Length:   141
Splice Variants Targeted:   ENST00000541683 ENST00000424501 ENST00000491310 ENST00000350997 ENST00000544309 ENST00000448607 ENST00000421879 ENST00000540767 ENST00000544696 ENST00000539419 ENST00000545986 ENST00000545245 ENST00000545405 ENST00000542506 ENST00000433932 ENST00000473263 ENST00000466716
LNCipedia Splice Variants Targeted:   lnc-C11orf10-2:1 lnc-C11orf10-2:2

Gene Information

The protein encoded by this gene is a member of the fatty acid desaturase (FADS) gene family. Desaturase enzymes regulate unsaturation of fatty acids through the introduction of double bonds between defined carbons of the fatty acyl chain. FADS family members are considered fusion products composed of an N-terminal cytochrome b5-like domain and a C-terminal multiple membrane-spanning desaturase portion both of which are characterized by conserved histidine motifs. This gene is clustered with family members FADS1 and FADS2 at 11q12-q13.1; this cluster is thought to have arisen evolutionarily from gene duplication based on its similar exon/intron organization. [provided by RefSeq Jul 2008]

Gene Symbol:   FADS1
Gene Name:   fatty acid desaturase 1
Aliases:   D5D, FADS6, FADSD5, FLJ38956, FLJ90273, LLCDL1, TU12
RefSeq:   NC_000011.9 NT_167190.1
Ensembl:   ENSG00000149485
LNCipedia:   lnc-C11orf10-2
Entrez:   3992
Chromosome Mapping:   11q12.2-q13.1
Gene Strand:   Not Available

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10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download