PrimePCR™ lncRNA SYBR® Green Assay:DDX21, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression.

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qhsaLEP0210658

List Price:    $169.00
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PrimePCR™ IncRNA PreAmp for SYBR® Green Assay: DDX21, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ IncRNA Template for SYBR® Green Assay: DDX21, Human
Reaction:     4 billion copies/200 µl desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   lncRNA Expression
Unique Assay ID:   qhsaLED0133418
Assay Design:   exonic
Chromosome Location:   chr10:68959828-68959950question
Amplicon Length:   93
Refseq Transcripts Amplified:   NM_004728,XM_011540336,NM_001256910,XM_017016910
Splice Variants Targeted:   ENST00000354185 ENST00000620315
LNCipedia Splice Variants Targeted:   lnc-DDX21-1:1

Gene Information

DEAD box proteins characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD) are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation nuclear and mitochondrial splicing and ribosome and spliceosome assembly. Based on their distribution patterns some members of this family are believed to be involved in embryogenesis spermatogenesis and cellular growth and division. This gene encodes a DEAD box protein which is an antigen recognized by autoimmune antibodies from a patient with watermelon stomach disease. This protein unwinds double-stranded RNA folds single-stranded RNA and may play important roles in ribosomal RNA biogenesis RNA editing RNA transport and general transcription. [provided by RefSeq Jul 2008]

Gene Symbol:   DDX21
Gene Name:   DEAD (Asp-Glu-Ala-Asp) box polypeptide 21
Aliases:   DKFZp686F21172, GUA, GURDB, RH-II/GU, RH-II/GuA
RefSeq:   NC_000010.10 NG_017061.1 NT_030059.13
Ensembl:   ENSG00000165732
LNCipedia:   lnc-DDX21-1
Entrez:   9188
Chromosome Mapping:   10q21
Gene Strand:   Not Available

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10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download