PrimePCR™ lncRNA SYBR® Green Assay:DNMT3B, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression.

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qhsaLEP0181854

List Price:    $169.00
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PrimePCR™ IncRNA PreAmp for SYBR® Green Assay: DNMT3B, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ IncRNA Template for SYBR® Green Assay: DNMT3B, Human
Reaction:     4 billion copies/200 µl desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   lncRNA Expression
Unique Assay ID:   qhsaLED0104614
Assay Design:   exonic
Chromosome Location:   chr20:32809098-32809323question
Amplicon Length:   196
Refseq Transcripts Amplified:   NM_006892,NM_175848,NM_175849,NM_001207055,NM_001207056,NM_175850,XM_011528653,XR_936511
Splice Variants Targeted:   ENST00000353855 ENST00000201963 ENST00000328111 ENST00000348286
LNCipedia Splice Variants Targeted:   lnc-DNMT3B-1:1

Gene Information

CpG methylation is an epigenetic modification that is important for embryonic development imprinting and X-chromosome inactivation. Studies in mice have demonstrated that DNA methylation is required for mammalian development. This gene encodes a DNA methyltransferase which is thought to function in de novo methylation rather than maintenance methylation. The protein localizes primarily to the nucleus and its expression is developmentally regulated. Mutations in this gene cause the immunodeficiency-centromeric instability-facial anomalies (ICF) syndrome. Eight alternatively spliced transcript variants have been described. The full length sequences of variants 4 and 5 have not been determined. [provided by RefSeq May 2011]

Gene Symbol:   DNMT3B
Gene Name:   DNA (cytosine-5-)-methyltransferase 3 beta
Aliases:   ICF, M.HsaIIIB
RefSeq:   NC_000020.10 NG_007290.1 NT_011362.10
Ensembl:   ENSG00000088305
LNCipedia:   lnc-DNMT3B-1
Entrez:   1789
Chromosome Mapping:   20q11.2
Gene Strand:   Not Available

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10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download