PrimePCR™ SYBR® Green Assay: CRIP1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCJP0034593

List Price:    $174.00
Your Price:   Log In
Quantity:  
 
Add to Custom Plate

PrimePCR™ PreAmp for SYBR® Green Assay: CRIP1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: CRIP1, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCJD0034565
Assay Design:   Exon-exon junction
Chromosome Location:   14:105954708-105955033question
Amplicon Length:   150

Gene Information

Cysteine-rich intestinal protein (CRIP) belongs to the LIM/double zinc finger protein family members of which include cysteine- and glycine-rich protein-1 (CSRP1; MIM 123876) rhombotin-1 (RBTN1; MIM 186921) rhombotin-2 (RBTN2; MIM 180385) and rhombotin-3 (RBTN3; MIM 180386). CRIP may be involved in intestinal zinc transport (Hempe and Cousins 1991 [PubMed 1946385]).[supplied by OMIM Mar 2008]

Gene Symbol:   CRIP1
Gene Name:   cysteine-rich protein 1 (intestinal)
Aliases:   CRHP, CRIP, CRP1, FLJ40971
RefSeq:   NC_000014.8 NT_026437.12
Ensembl:   ENSG00000213145
Entrez:   1396
Chromosome Mapping:   14q32.33

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998800
y-intercept 36.130000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download