PrimePCR™ Probe Assay: CDSN, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: CDSN, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: CDSN, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0041522
Assay Design:   Intron-spanning
Chromosome Location:   6:31085234-31088136question
Amplicon Length:   68
Splice Variants Targeted:   ENST00000450791 ENST00000204726 ENST00000406341 ENST00000407969 ENST00000302463 ENST00000399686 ENST00000402198 ENST00000402466 ENST00000360105 ENST00000358552 ENST00000445893 ENST00000397708 ENST00000291688 ENST00000375241 ENST00000265659 ENST00000375236 ENST00000031146 ENST00000429147 ENST00000393946 ENST00000457875 ENST00000418599 ENST00000259726 ENST00000376288 ENST00000599216 ENST00000595360 ENST00000303210 ENST00000436220 ENST00000424361 ENST00000419828 ENST00000427895 ENST00000393926 ENST

Gene Information

This gene encodes a protein found in corneodesmosomes which localize to human epidermis and other cornified squamous epithelia. During maturation of the cornified layers the protein undergoes a series of cleavages which are thought to be required for desquamation. The gene is located in the major histocompatibility complex (MHC) class I region on chromosome 6. [provided by RefSeq Jul 2008]

Gene Symbol:   CDSN
Gene Name:   corneodesmosin
Aliases:   D6S586E, HTSS, S
RefSeq:   NC_000006.11 NG_012192.1 NG_021348.1 NT_007592.15 NT_113891.2 NT_167245.1 NT_167246.1 NT_167247.1 NT_167248.1
Ensembl:   ENSG00000204539
Entrez:   1041
Chromosome Mapping:   6p21.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998700
y-intercept 36.290000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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