PrimePCR™ Probe Assay: RHCE, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: RHCE, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: RHCE, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0041503
Assay Design:   Intron-spanning
Chromosome Location:   1:25688942-25697026question
Amplicon Length:   142
Splice Variants Targeted:   ENST00000328664 ENST00000294413 ENST00000340849 ENST00000349320 ENST00000527747 ENST00000346452 ENST00000349438 ENST00000455194 ENST00000374352 ENST00000243186 ENST00000425135

Gene Information

The Rh blood group system is the second most clinically significant of the blood groups second only to ABO. It is also the most polymorphic of the blood groups with variations due to deletions gene conversions and missense mutations. The Rh blood group includes this gene which encodes both the RhC and RhE antigens on a single polypeptide and a second gene which encodes the RhD protein. The classification of Rh-positive and Rh-negative individuals is determined by the presence or absence of the highly immunogenic RhD protein on the surface of erythrocytes. A mutation in this gene results in amorph-type Rh-null disease. Alternative splicing of this gene results in four transcript variants encoding four different isoforms. [provided by RefSeq Jul 2008]

Gene Symbol:   RHCE
Gene Name:   Rh blood group, CcEe antigens
Aliases:   CD240CE, MGC103977, RH, RH30A, RHC, RHE, RHIXB, RHPI, Rh4, RhIVb(J), RhVI, RhVIII
RefSeq:   NC_000001.10 NG_003255.2 NG_009208.2 NT_004610.19
Ensembl:   ENSG00000188672
Entrez:   6006
Chromosome Mapping:   1p36.11

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 36.420000
Efficiency 95

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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