PrimePCR™ Probe Assay: GCLC, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0041350
Assay Design:   Intron-spanning
Chromosome Location:   6:53385585-53387257question
Amplicon Length:   189
Splice Variants Targeted:   ENST00000229416 ENST00000514004 ENST00000513939 ENST00000514933

Gene Information

Glutamate-cysteine ligase also known as gamma-glutamylcysteine synthetase is the first rate-limiting enzyme of glutathione synthesis. The enzyme consists of two subunits a heavy catalytic subunit and a light regulatory subunit. This locus encodes the catalytic subunit while the regulatory subunit is derived from a different gene located on chromosome 1p22-p21. Mutations at this locus have been associated with hemolytic anemia due to deficiency of gamma-glutamylcysteine synthetase and susceptibility to myocardial infarction.[provided by RefSeq Oct 2010]

Gene Symbol:   GCLC
Gene Name:   glutamate-cysteine ligase, catalytic subunit
Aliases:   GCL, GCS, GLCL, GLCLC
RefSeq:   NC_000006.11 NG_012071.1 NT_007592.15
Ensembl:   ENSG00000001084
Entrez:   2729
Chromosome Mapping:   6p12

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999800
y-intercept 35.520000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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